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With the rise of Arabidopsis as a study system, and notably genome sequencing, the pathway and its genes were characterized at the molecular genetic level, revealing (or confirming) that all four enzymes were encoded by more than one gene, thus explaining the presence of the enzyme activities in several compartments (Table 1)

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Dopamine transporter binding was evaluated by using DaTscan imaging (DTscan is a radiopharmaceutical used in a brain scan (SPECT), to visualize striatal dopamine transporters)