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Using the MTT assay (a standard test for cell viability), they measured whether AHK-Cu made these cells multiply faster

PMID: 16330726

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Exposure to physiological levels of nitrosative stress showed no difference in the magnitude of GSH:GSSG from cells derived from children with autistic disorder compared to healthy controls, however, the baseline GSH:GSSG was significantly lower (by 30%) in cells from children with autistic disorder